Located on a 70-acre nature reserve in Wisconsin's Lower Sugar River Valley, Three Waters Reserve invites visitors to explore its public trails and enjoy passive nature observation. The trails are open year-round from dawn until dusk and do not allow motorized vehicles.

Home to five conservation organizations, including the Southern Wisconsin Land Conservancy, the Lower Sugar River Watershed Association, the Lower Sugar River Field Station, the Applied Ecological Institute, and Culinary Nirvana, Three Waters Reserve offers community and educational events such as Lunch and Learn programs and guided trail walks.