Eureka Maple Woods lies on a steep, north-facing slope above Timber Coulee Creek, a tributary of Coon Creek. The forest is dominated by small to medium sized sugar maple and basswood, along with red oak, yellow birch, white ash and red elm. Large trees are uncommon. However, the herbaceous flora is exceptionally rich, containing such uncommon plants as squirrel corn, twinleaf, Goldie's fern, narrow-leaved spleenwort, walking fern, leafcup, and adam and eve orchid. Common mesic ground layer species are abundant, providing a tremendous springtime floral display. The shrub layer is also diverse with witch hazel, leatherwood, hazelnut, bladdernut, viburnums, and dogwoods. Bird species include several species sensitive to forest fragmentation such as cerulean warbler (Dendroica cerulea), Acadian flycatcher (Empidonax virescens), ovenbird, yellow-throated vireo, American redstart, blue-gray gnatcatcher, and wood thrush.